Question/ Problem: The Effect of Various Antibiotics on Bacterial Growth.

Title: “Antibacterial Properties of Selected Hand Sanitizers”.

Hypothesis- If 5 recommended brands of hand sanitizers and Protex Antibacterial soap are added to cultivated bacteria, then Protex will work best in killing the bacteria.

Variables:

IV- Selected Hand sanitizers and Protex Antibacterial Soap.

DP- The number of the bacteria left over.

Constants- Amount of hand sanitizer, the size of the bacteria culture, environment, atmosphere, temperature, Petri dishes, placement, and the spaces between the Petri dishes.

Controlled Group- Protex Antibacterial Soap and water applied to bacteria.

Experimental Group- Bacteria exposed to selected brands of hand sanitizers.

Procedure:

1. Label the back of 6 agar plates correspondingly to what will be used on that slide. (Ex: AO 1, PR 1 etc.)
2. Cultivate bacteria cultures on the agar plates for all 6 plates.
3. After the bacteria have fully grown, smear the hand sanitizers and soap evenly on their corresponding plates.
4. Keep the agar plates in the same environment for an hour and observe the changes.
5. See how many bacteria have been eliminated and how many survived.
6. Repeat the same procedure 3 times in order to have accurate observations.
7. Based on your findings and observations, make an acceptable conclusion as to which hand sanitizer will work best in killing/declining the bacteria.

Materials-

1.                  Agar plates

2.                  Bacteria culture

3.                  Hand sanitizers (Avant, Original Germ-X, Purell, Wet Ones, EO),

4.                  Soup (Protex antibacterial soap)

5.                  Distilled water

6.                  Basic gloves